{"id":690,"date":"2024-03-08T16:11:41","date_gmt":"2024-03-08T21:11:41","guid":{"rendered":"https:\/\/blog.richmond.edu\/ti3d\/?p=690"},"modified":"2024-03-12T12:58:02","modified_gmt":"2024-03-12T16:58:02","slug":"electrophoresis-comb-and-holder","status":"publish","type":"post","link":"https:\/\/blog.richmond.edu\/ti3d\/2024\/03\/08\/electrophoresis-comb-and-holder\/","title":{"rendered":"Electrophoresis Comb and Holder"},"content":{"rendered":"<p>Gel <span class=\"searchHighlight\">electrophoresis<\/span> is a lab method used to separate mixtures of DNA, RNA, or proteins according to molecular size, and it is used in both teaching and scholarship here at UR. Dr. Omar Quintero needed help designing <span class=\"searchHighlight\">electrophoresis<\/span> combs (and their associated holder) that have more teeth than available elsewhere commercially. That resulted in a design that uses <a title=\"https:\/\/openscad.org\/\" contenteditable=\"false\" href=\"https:\/\/openscad.org\" target=\"_blank\" rel=\"noopener\">OpenSCAD<\/a>, a CAD tool that employs scripting techniques to build 3D objects. The design succeeded, and Dr. Quintero was able to print them with his own 3D printer. Since the design is based on a script, it can be used and modified by others in the biology community.<\/p>\n<p>&nbsp;<\/p>\n\n\t\t<style type=\"text\/css\">\n\t\t\t#gallery-1 {\n\t\t\t\tmargin: auto;\n\t\t\t}\n\t\t\t#gallery-1 .gallery-item {\n\t\t\t\tfloat: left;\n\t\t\t\tmargin-top: 10px;\n\t\t\t\ttext-align: center;\n\t\t\t\twidth: 33%;\n\t\t\t}\n\t\t\t#gallery-1 img {\n\t\t\t\tborder: 2px solid #cfcfcf;\n\t\t\t}\n\t\t\t#gallery-1 .gallery-caption {\n\t\t\t\tmargin-left: 0;\n\t\t\t}\n\t\t\t\/* see gallery_shortcode() in wp-includes\/media.php *\/\n\t\t<\/style>\n\t\t<div id='gallery-1' class='gallery galleryid-690 gallery-columns-3 gallery-size-thumbnail'><dl class='gallery-item'>\n\t\t\t<dt class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/blog.richmond.edu\/ti3d\/2024\/03\/08\/electrophoresis-comb-and-holder\/image005\/'><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/blog.richmond.edu\/ti3d\/files\/2024\/03\/image005-300x300.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-692\" \/><\/a>\n\t\t\t<\/dt>\n\t\t\t\t<dd class='wp-caption-text gallery-caption' id='gallery-1-692'>\n\t\t\t\tfinal printed comb and comb holder\n\t\t\t\t<\/dd><\/dl><dl class='gallery-item'>\n\t\t\t<dt class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/blog.richmond.edu\/ti3d\/2024\/03\/08\/electrophoresis-comb-and-holder\/screenshot-2024-03-08-at-4-01-43-pm\/'><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/blog.richmond.edu\/ti3d\/files\/2024\/03\/Screenshot-2024-03-08-at-4.01.43\u202fPM-300x300.png\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-693\" \/><\/a>\n\t\t\t<\/dt>\n\t\t\t\t<dd class='wp-caption-text gallery-caption' id='gallery-1-693'>\n\t\t\t\tOpenSCAD interface with one of the combs\n\t\t\t\t<\/dd><\/dl><dl class='gallery-item'>\n\t\t\t<dt class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/blog.richmond.edu\/ti3d\/2024\/03\/08\/electrophoresis-comb-and-holder\/screenshot-2024-03-08-at-4-10-27-pm\/'><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/blog.richmond.edu\/ti3d\/files\/2024\/03\/Screenshot-2024-03-08-at-4.10.27\u202fPM-300x300.png\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-691\" \/><\/a>\n\t\t\t<\/dt>\n\t\t\t\t<dd class='wp-caption-text gallery-caption' id='gallery-1-691'>\n\t\t\t\tSketchup app showing 3 of the combs and hodler\n\t\t\t\t<\/dd><\/dl><br style=\"clear: both\" \/><dl class='gallery-item'>\n\t\t\t<dt class='gallery-icon landscape'>\n\t\t\t\t<a href='https:\/\/blog.richmond.edu\/ti3d\/2024\/03\/08\/electrophoresis-comb-and-holder\/maxresdefault\/'><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/blog.richmond.edu\/ti3d\/files\/2024\/03\/maxresdefault-300x300.jpg\" class=\"attachment-thumbnail size-thumbnail\" alt=\"\" aria-describedby=\"gallery-1-763\" \/><\/a>\n\t\t\t<\/dt>\n\t\t\t\t<dd class='wp-caption-text gallery-caption' id='gallery-1-763'>\n\t\t\t\tcomb being manually used with a gel\n\t\t\t\t<\/dd><\/dl>\n\t\t\t<br style='clear: both' \/>\n\t\t<\/div>\n\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Gel electrophoresis is a lab method used to separate mixtures of DNA, RNA, or proteins according to molecular size, and it is used in both teaching and scholarship here at UR. Dr. Omar Quintero needed help designing electrophoresis combs (and their associated holder) that have more teeth than available elsewhere commercially. That resulted in a [&hellip;]<\/p>\n","protected":false},"author":602,"featured_media":692,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[9306,9297,9300,508,9296],"tags":[],"class_list":["post-690","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ur","category-design","category-printing","category-research","category-sketchup"],"jetpack_featured_media_url":"https:\/\/blog.richmond.edu\/ti3d\/files\/2024\/03\/image005.jpg","_links":{"self":[{"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/posts\/690","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/users\/602"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/comments?post=690"}],"version-history":[{"count":3,"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/posts\/690\/revisions"}],"predecessor-version":[{"id":765,"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/posts\/690\/revisions\/765"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/media\/692"}],"wp:attachment":[{"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/media?parent=690"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/categories?post=690"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.richmond.edu\/ti3d\/wp-json\/wp\/v2\/tags?post=690"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}